[Autolytic degradation of RNA: effect of autolysates and native preparations of pre-mRNA on the level of transcription in model systems in vitro and in vivo].

The effects of autolysates and native preparations of pre-mRNA on RNA biosynthesis in isolated rat liver nuclei in vitro and in mouse liver cells in vivo were studied. The correlation between the experimental data obtained in both test systems and the differences in the effects of native pre-mRNA preparations and their autolysates suggest that the autolytic degradation in vitro is a reflection of processes occurring in eukaryotic cells. The feasible role and significance of such fragments in the general scheme of transcription regulation are discussed.