de la Rosa F F, Palacián E, Castillo F
Rev Esp Fisiol. 1980 Sep;36(3):279-83.
Based on Lineweaver-Burk plots of the initial velocities, at different concentrations of NADH and nitrate, and product inhibition patterns, an Iso Ping Pong Bi Bi steady state kinetic mechanism is proposed for the spinach nitrate reductase. This mechanism incorporates the concept that the oxidized enzyme is present in two isomeric forms.
基于不同浓度烟酰胺腺嘌呤二核苷酸(NADH)和硝酸盐条件下初始反应速度的林-贝氏图以及产物抑制模式,提出了菠菜硝酸还原酶的有序双双乒乓稳态动力学机制。该机制纳入了氧化态酶以两种异构体形式存在的概念。