The nature of NADPH-dependent lipid peroxidation in rat kidney microsomes.

The NADPH-dependent lipid peroxidation activity of rat kidney microsomes was one-third of that of liver microsomes. This activity of kidney microsomes was inhibited by EDTA, cytochrome c, Co2+, Mn2+, Cr6+, and aminopyrine, in agreement with the results reported by several investigators for liver microsomes. However, the Km for NADPH, the effect of PCMB or NEM, and the inhibitory effect of carbon tetrachloride on lipid peroxidation of kidney microsomes were different from those in liver microsomes. These results reveal that the NADPH-dependent peroxidizing system of kidney microsomes is appreciably different from that of liver microsomes.