Trottier B, Fraser A R, Planet G, Ecobichon D J
Toxicology. 1980;17(1):29-38. doi: 10.1016/0300-483x(80)90024-4.
Male CD-strain rats (100--125 g) received fenitrothion by oral gavage (2.5, 5.0, 10.0 or 20.0 mg/kg/day) for 30 consecutive days. Control animals received equivalent volumes of taurocholate vehicle. Animals were killed at 8, 15, 22 and 30 days during treatment and at 8, 15, 29, 57 and 85 days after terminating treatment. The brain plasma and erythrocyte cholinesterases, hepatic and renal non-specific carboxylesterases were assayed. Serum biochemical markers of hepatic function were measured. Histologically stained sections of liver were examined by light microscopy. Fenitrothion, at doses between 2.5 and 10 mg/kg body wt, caused few observable deleterious effects to male rats except those effects on tissue esterases associated with organophosphorus esters. Only 8 of 36 animals receiving the 20.0 mg/kg dose died, all in the first week of treatment. A dose-dependent decrease in tissue esterase activity was observed which persisted throughout the entire treatment period. Periodic signs of overt toxicity were observed only in animals receiving 20 mg/kg/day. Tissue esterase activities returned to control levels within 1 or 2 weeks of stopping treatment. No significant treatment-related changes were observed by serum biochemistry or by light microscopy.
雄性CD品系大鼠（体重100 - 125克）连续30天经口灌胃给予杀螟硫磷（2.5、5.0、10.0或20.0毫克/千克/天）。对照动物给予等量的牛磺胆酸盐溶媒。在治疗期间的第8、15、22和30天以及终止治疗后的第8、15、29、57和85天处死动物。测定脑血浆和红细胞胆碱酯酶、肝和肾非特异性羧酸酯酶。检测肝功能的血清生化标志物。通过光学显微镜检查肝脏组织学染色切片。杀螟硫磷剂量在2.5至10毫克/千克体重之间时，除了对与有机磷酸酯相关的组织酯酶有影响外，对雄性大鼠几乎没有可观察到的有害影响。接受20.0毫克/千克剂量的36只动物中只有8只死亡，均在治疗的第一周。观察到组织酯酶活性呈剂量依赖性下降，且在整个治疗期间持续存在。仅在接受20毫克/千克/天的动物中观察到明显毒性的周期性体征。停止治疗后1至2周内组织酯酶活性恢复到对照水平。血清生化检查或光学显微镜检查未观察到与治疗相关的显著变化。
