Shaw J O
Am J Pathol. 1980 Nov;101(2):283-302.
Lung inflammation was induced in rabbits by intratracheal injections of chemotactic fragments obtained from zymosan-activated serum (CF-ZAS), and the route of vascular emigration and alveolar surface interaction of polymorphonuclear leukocytes (PMNs) and monocytes migrating into the lung was characterized by transmission (TEM) and scanning (SEM) electron-microscopic examination. Leukocytes migrated from capillaries and venules into the alveolar wall interstitium by adherence to the vascular endothelium and migration through the endothelial intracellular junction to attain a position between a reapposed endothelial cell junction and the vascular basement membrane. The cells then migrated into the interstitium through a narrow opening in the basement membrane. Leukocyte entrance into the alveolar space from the interstitium appeared to occur through small openings in the epithelial basement membrane at or near the Type I epithelial intercellular junction. Once in the alveolus, PMNs and macrophages demonstrated surface adherence and spreading along with evidence of migration, pseudopod extension, interalveolar pore transit, and retraction fiber formation. This study indicates the leukocyte influx into the alveolus in acute chemotactic-factor-induced inflammation is via a continuum of migrational activity, beginning at the pulmonary capillary endothelial surface and persisting on the alveolar epithelial surface.
通过气管内注射从酵母聚糖激活血清中获得的趋化片段（CF-ZAS）诱导兔肺部炎症，并用透射电子显微镜（TEM）和扫描电子显微镜（SEM）检查来表征多形核白细胞（PMN）和单核细胞迁移到肺中的血管迁移途径和肺泡表面相互作用。白细胞通过粘附于血管内皮并通过内皮细胞内连接迁移，从毛细血管和小静脉迁移到肺泡壁间质，到达重新贴合的内皮细胞连接和血管基底膜之间的位置。然后细胞通过基底膜上的狭窄开口迁移到间质中。白细胞从间质进入肺泡腔似乎是通过I型上皮细胞间连接处或其附近的上皮基底膜上的小开口发生的。一旦进入肺泡，PMN和巨噬细胞就表现出表面粘附和铺展，同时还有迁移、伪足延伸、肺泡间孔通过和收缩纤维形成的证据。这项研究表明，在急性趋化因子诱导的炎症中，白细胞流入肺泡是通过一系列连续的迁移活动，始于肺毛细血管内皮表面并持续于肺泡上皮表面。
