Damiano V V, Cohen A, Tsang A L, Batra G, Petersen R
Am J Pathol. 1980 Aug;100(2):349-64.
The appearance of neutrophils in the alveoli of the lung is a common result of pulmonary infection, although the route of neutrophil migration across the alveolar wall has not been demonstrated in normal animals. Recently, however, several methods of stimulating the influx of neutrophils into alveoli have been developed. In the present study, the lung wash model was used to attract large numbers of neutrophils into the airways in order to identify the site of influx of alveolar neutrophils. The combined use of scanning and transmission electron microscopy has made it possible to visualize the migration of neutrophils from the capillaries, through the interstitial spaces, and through the epithelium at the junctions between Type I and Type II cells.
中性粒细胞出现在肺的肺泡中是肺部感染的常见结果，尽管在正常动物中尚未证实中性粒细胞穿过肺泡壁的迁移途径。然而，最近已经开发出几种刺激中性粒细胞流入肺泡的方法。在本研究中，使用肺灌洗模型将大量中性粒细胞吸引到气道中，以确定肺泡中性粒细胞的流入部位。扫描电子显微镜和透射电子显微镜的联合使用使得观察中性粒细胞从毛细血管，穿过间质间隙，以及穿过I型和II型细胞之间连接处的上皮细胞的迁移成为可能。
