Widdicombe J H, Welsh M J
Am J Physiol. 1980 Sep;239(3):C112-7. doi: 10.1152/ajpcell.1980.239.3.C112.
We have measured the fluxes of 82Br or 131I across short-circuited dog tracheal epithelium following complete replacement of chloride in the bathing medium by either bromide or iodide. In addition, we have partly replaced the bathing chloride with bromide, iodide, or thiocyanate and studied the effects of these anions on 36Cl fluxes. Our results show that the affinity sequence for the chloride transport process is I > Br congruent to Cl, that Br is transported, but that I is not. Thiocyanate is a potent inhibitor of chloride transport, and its effects are greater than those predicted by a simple competitive interaction with chloride for transport sites.
在将浴液中的氯化物完全替换为溴化物或碘化物后，我们测量了82Br或131I通过短路犬气管上皮的通量。此外，我们用溴化物、碘化物或硫氰酸盐部分替代浴液中的氯化物，并研究了这些阴离子对36Cl通量的影响。我们的结果表明，氯化物转运过程的亲和力顺序为I > Br ≈ Cl，Br可被转运，但I不能。硫氰酸盐是氯化物转运的有效抑制剂，其作用大于与氯化物竞争转运位点所预测的简单竞争作用。
