Al-Bazzaz F, Yadava V P, Westenfelder C
Am J Physiol. 1981 Feb;240(2):F101-5. doi: 10.1152/ajprenal.1981.240.2.F101.
We tested the effect of prostaglandins PGF2 alpha and PGE1 on the transport of 36Cl and 22Na by canine tracheal epithelium. Sheets of epithelium were mounted in Ussing chambers and short-circuited. Addition of PGF2 alpha to the mucosal side resulted in an increase of net Cl secretion from 0.71 +/- 0.41 to 2.40 +/- 0.67 mu eq . cm-2 . h-1 without significant effect on net Na absorption. Prostaglandin E1 on the mucosal side increased net Cl secretion from 1.36 +/- 0.31 to 2.69 +/- 0.35 and decreased Na absorption from 0.87 +/- 0.16 to 0.49 +/- 0.09. Indomethacin significantly depressed net Cl secretion from 1.36 +/- 0.36 to 0.57 +/- 0.22. Subsequent addition of PGE1 augmented net Cl secretion to 3.88 +/- 0.75. PGE1 did not enhance [14C]mannitol fluxes across this epithelium. Cellular levels of cAMP increased in response to PGE1 from 130 +/- 12.7 to 642 +/- 33.4 pmol . mg prot-1 . 10 min-1, whereas PGF2 alpha had no effect. These data suggest that although effects of PGF2 alpha and PGE1 are similar as pertains to net Cl secretion, they differ in their effects on Na transport and their capacity to increase cAMP levels. Alterations in Cl and Na transport in response to PGE1 are likely to be mediated, at least in part, by the adenylate cyclase-cAMP system. Furthermore, endogenous prostaglandins may have an important regulatory role in ion transport by airways epithelium.
我们测试了前列腺素PGF2α和PGE1对犬气管上皮细胞转运36Cl和22Na的影响。将上皮细胞片安装在尤斯灌流小室中并使其短路。在黏膜侧添加PGF2α导致净氯分泌从0.71±0.41增加到2.40±0.67μeq·cm-2·h-1，而对净钠吸收无显著影响。黏膜侧的前列腺素E1使净氯分泌从1.36±0.31增加到2.69±0.35，并使钠吸收从0.87±0.16降低到0.49±0.09。吲哚美辛显著抑制净氯分泌，从1.36±0.36降至0.57±0.22。随后添加PGE1使净氯分泌增加到3.88±0.75。PGE1并未增强[14C]甘露醇通过该上皮细胞的通量。细胞内cAMP水平因PGE1从130±12.7增加到642±33.4 pmol·mg蛋白-1·10 min-1，而PGF2α无此作用。这些数据表明，尽管PGF2α和PGE1在净氯分泌方面作用相似，但它们对钠转运的影响以及增加cAMP水平的能力有所不同。PGE1引起的氯和钠转运变化可能至少部分由腺苷酸环化酶 - cAMP系统介导。此外，内源性前列腺素可能在气道上皮细胞的离子转运中起重要调节作用。
