Landy A, Hsu P L, Ross W, Buraczynska M
Am J Trop Med Hyg. 1980 Sep;29(5 Suppl):1099-106. doi: 10.4269/ajtmh.1980.29.1099.
Site-specific integrative recombination in bacteriophage lambda involves unequal partners. The minimal phage att site is composed of approximately 240 base pairs and has four distinct Int binding sites that differ in size and response to heparin challenge. There appear to be two size classes of Int binding sites, approximately 30-35 base pairs and 15 base pairs. The sites at the common core and in the P' arm are of the former class. Two sites in the P arm are of the latter class. Thus far, three of the four sites have been shown to be necessary for att site function. In contrast, the minimal sequence required for a phage att site partner (such as the bacterial att site) may not be much larger than the 15 base pair common core. We have suggested a model in which integrative recombination involves two unequal partners; accordingly the phage att site is referred to as the "donor" and the bacterial att site, or its analogue, is referred to as the "recipient."
噬菌体λ中的位点特异性整合重组涉及不相等的伙伴。最小的噬菌体附着位点（att位点）由大约240个碱基对组成，有四个不同的整合酶（Int）结合位点，其大小和对肝素攻击的反应各不相同。似乎存在两种大小类别的Int结合位点，大约30 - 35个碱基对和15个碱基对。共同核心区和P'臂中的位点属于前一类。P臂中的两个位点属于后一类。到目前为止，四个位点中的三个已被证明对att位点功能是必需的。相比之下，噬菌体att位点伙伴（如细菌att位点）所需的最小序列可能不比15个碱基对的共同核心大多少。我们提出了一个模型，其中整合重组涉及两个不相等的伙伴；因此，噬菌体att位点被称为“供体”，细菌att位点或其类似物被称为“受体”。
