Esbenshade K L, Clegg E D
Am J Vet Res. 1980 Jul;41(7):1137-40.
A procedure is described for incubation and recopvery of sperm from the female reproductive tract. Ova which were placed in Teflon capsules and surgically inserted into ligated uterine segments of gilts, were incubated with sperm and examined to determine whether the acrosome reaction was induced. Ova were recovered at 30-minute intervals for 2 hours and examined by electron microscopy. When 6 to 12 X 10(9) sperm were placed into the ligated uterine segments, 80% to 85% of the sperm were recovered. The acrosome reaction, with the characteristic vesiculation of the plasma membrane and outer acrosomal membrane was detected in sperm at the surface of the ova after incubation for 60 or 90 minutes. Approximately 5% of the sperm outside the capsules showed similar membrane vesiculation after those time intervals.
本文描述了一种从雌性生殖道中孵育和回收精子的方法。将卵子置于聚四氟乙烯胶囊中，通过手术植入后备母猪结扎的子宫段，与精子一起孵育，并检查是否诱导了顶体反应。每隔30分钟回收一次卵子，持续2小时，并通过电子显微镜检查。当将6至12×10⁹个精子放入结扎的子宫段时，80%至85%的精子被回收。孵育60或90分钟后，在卵子表面的精子中检测到具有质膜和顶体外膜特征性水泡化的顶体反应。在这些时间间隔后，胶囊外约5%的精子显示出类似的膜水泡化。
