Talbot P, Franklin L E
J Exp Zool. 1976 Nov;198(2):163-75. doi: 10.1002/jez.1401980206.
The morphology and kinetics of the normal acrosome reaction were examined in vitro using hamster sperm incubated in detoxified sera. The reaction involved either swelling and elevation or crenulation and fragmentation of the acrosomal cap. Swelling and elevation occurred during both normal and degenerative reactions, as reported by others. Crenulation with subsequent fragmentation of the cap was observed during normal reactions. Early crenulation of the acrosome could be induced by cold shock (5 degrees C, 25 minutes), but this did not decrease the incubation time required (at 37 degrees C) for completion of the normal reaction. In appropriate sera, the occurrence of normal and degenerative acrosome reactions in motile sperm was significantly separated in time to study the reactions independently. The duration of the normal reaction, i.e., the time between the first morphological change in the acrosome (initiation)until the actual detachment of the cap (termination) was estimated to be 20 minutes. Saline dilution of these sera delayed initiation of the reaction and increased the duration of the reaction once it had started. Data from cold-shock and serum dilution experiments indicate that the mechanisms which govern the initiation and termination of the normal reaction are independently variable, and further suggest that initiation involves a change in membrane permeability and that termination includes membrane vesiculation.
利用在解毒血清中孵育的仓鼠精子，在体外研究了正常顶体反应的形态学和动力学。该反应包括顶体帽的肿胀和隆起或皱缩和破碎。如其他人所报道的，肿胀和隆起在正常反应和退化反应中均会发生。在正常反应过程中观察到顶体帽出现皱缩并随后破碎。顶体的早期皱缩可由冷休克（5摄氏度，25分钟）诱导，但这并未减少正常反应完成所需的（37摄氏度下的）孵育时间。在合适的血清中，活动精子中正常顶体反应和退化顶体反应的发生在时间上有明显区分，从而能够独立研究这些反应。正常反应的持续时间，即从顶体首次出现形态变化（起始）到帽实际脱离（终止）的时间估计为20分钟。这些血清用盐水稀释会延迟反应的起始，并在反应开始后增加反应的持续时间。冷休克和血清稀释实验的数据表明，控制正常反应起始和终止的机制是独立可变的，并且进一步表明起始涉及膜通透性的变化，而终止包括膜囊泡化。
