Johnson A, Malik A B
Am Rev Respir Dis. 1980 Oct;122(4):561-6. doi: 10.1164/arrd.1980.122.4.561.
We studied granulocyte entrapment in the lung after pulmonary microembolization to see if it contributes to the increase in extravascular water content. The lungs were embolized by intravenous injection of nonsiliconized glass beads (100 micrometers in diameter) in normal dogs and dogs made granulocytopenic by daily injections of hydroxyurea. Hydroxyurea did not significantly alter the platelet and fibrinogen concentrations. Control and treated dogs were embolized to the same degree, which resulted in similar increases in pulmonary arterial pressure and pulmonary vascular resistance. The extravascular lung water content/bloodless dry lung weight (W/D) ratios in the control and treated groups after embolization were compared with the W/D ratios in the control and treated nonembolized groups, respectively. The W/D ratio increased from the value of 2.84 +/- 0.22 ml/g in the control group to 4.54 +/- 0.19 ml/g at 75 min after embolization (60% increase) (p < 0.05). However, the W/D ratio in the control-granulocytopenic group of 3.54 +/- 0.19 ml/g was not significantly different from the value of 4.09 +/- 0.27 ml/g in the granulocytopenic group after embolization. Because embolization after granulocytopenia produced only a 16% increase in the extravascular lung water content, compared with a 60% increase in the treated group after the same degree of embolization, the results suggested that granulocyte entrapment in the lung after microembolization contributes to the development of pulmonary edema.
我们研究了肺微血管栓塞后粒细胞在肺内的滞留情况，以确定其是否会导致血管外含水量增加。通过静脉注射非硅化玻璃珠（直径100微米）对正常犬和每日注射羟基脲致粒细胞减少的犬进行肺栓塞。羟基脲并未显著改变血小板和纤维蛋白原浓度。对照犬和处理犬的栓塞程度相同，导致肺动脉压和肺血管阻力出现相似程度的升高。分别将栓塞后对照组和处理组的血管外肺含水量/无血干肺重量（W/D）比值与未栓塞的对照组和处理组的W/D比值进行比较。对照组的W/D比值从2.84±0.22 ml/g增加至栓塞后75分钟时的4.54±0.19 ml/g（增加60%）（p<0.05）。然而，粒细胞减少对照组的W/D比值为3.54±0.19 ml/g，与栓塞后粒细胞减少组4.09±0.27 ml/g的值无显著差异。由于粒细胞减少后进行栓塞仅使血管外肺含水量增加16%，而相同程度栓塞后处理组增加60%，结果表明微血管栓塞后粒细胞在肺内的滞留促进了肺水肿的发展。
