Beal A M
Aust J Biol Sci. 1980 May;33(2):197-204. doi: 10.1071/bi9800197.
Salivary flow rate and the concentrations of electrolytes in parotid saliva and arterial plasma from anaesthetized sodium-replete sheep were measured before, during and after ipsilateral intracaroitd infusion of acetylcholine at 10 nmol min-1 to ascertain whether anaesthesia altered the relation between salivary flow and sodium concentration. The potential difference (PD) between the lumen of the parotid duct and the vascular system was also measured. Concentrations of salivary sodium and phosphate were decreased and potassium concentration and total CO2 content were increased when rate of salivary flow was increased by acetylcholine infusion. Salivary chloride concentration was reduced in five experiments and increased in three experiments when flow rate was elevated. Thus the flow-composition relations of parotid saliva from anaesthetized sheep were essentially the same as those for saliva from conscious animals. The PD between the lumen of the parotid duct and blood at resting flow rates was 9.4 +/- 1.07 mV, lumen negative. At high flow rates, stimulated by acetylcholine infusion, the PD increased to 21.9 +/- 2.20 mV, lumen negative. This increase in PD of the duct epithelium appeared to depend on changes in the composition of saliva arriving at the site of potential measurement.
在麻醉的、钠补充充足的绵羊中，于同侧颈内动脉以10 nmol·min-1的速率输注乙酰胆碱之前、期间和之后，测量唾液流速以及腮腺唾液和动脉血浆中电解质的浓度，以确定麻醉是否改变了唾液流速与钠浓度之间的关系。还测量了腮腺导管腔与血管系统之间的电位差（PD）。当通过输注乙酰胆碱增加唾液流速时，唾液钠和磷酸盐浓度降低，钾浓度和总二氧化碳含量增加。当流速升高时，在五个实验中唾液氯浓度降低，在三个实验中升高。因此，麻醉绵羊腮腺唾液的流速-成分关系与清醒动物的唾液基本相同。腮腺导管腔与血液在静息流速下的PD为9.4±1.07 mV，管腔为负。在高流速下，由乙酰胆碱输注刺激，PD增加到21.9±2.20 mV，管腔为负。导管上皮细胞PD的这种增加似乎取决于到达电位测量部位的唾液成分的变化。
