Brunner R, Zimmermann P, Klussmann F W
Cell Tissue Res. 1980;212(1):73-81. doi: 10.1007/BF00234034.
Evans blue was injected into either the medial and lateral gastrocnemius or the soleus muscle of adult albino rats to mark retrogradely the corresponding motoneurones in the spinal cord. The labelled motoneurones were identified by the red fluorescence of their perikarya in the ventral horn of segments L4--L6. In addition, a monosynaptic reflex action potential was recorded only in the ventral roots L4--L6 after stimulation of the nerves to the medial and lateral gastrocnemius and the soleus muscle. Excitability and reflex latencies of labelled and unlabelled motoneurones of segment L4--L6 as well as the conduction velocities of their axons were measured. Apart from a small but statistically nonsignificant increase in excitability, no functional differences were found between labelled and control neurones. Thus, retrograde labelling of motoneurones with Evans blue prior to performing electrophysiological experiments has the advantage that the marked motoneurones can be identified under the fluorescence microscope without the need of additional staining or fixation.
将伊文思蓝注入成年白化大鼠的腓肠肌内侧头、外侧头或比目鱼肌，以逆行标记脊髓中相应的运动神经元。通过其胞体在L4-L6节段腹角的红色荧光来识别标记的运动神经元。此外，在刺激腓肠肌内侧头、外侧头和比目鱼肌的神经后，仅在L4-L6腹根记录到单突触反射动作电位。测量了L4-L6节段标记和未标记运动神经元的兴奋性、反射潜伏期及其轴突的传导速度。除了兴奋性有小幅度但在统计学上无显著意义的增加外，标记神经元和对照神经元之间未发现功能差异。因此，在进行电生理实验之前用伊文思蓝逆行标记运动神经元的优点是，无需额外染色或固定，即可在荧光显微镜下识别标记的运动神经元。
