一种用于检测血浆总非酯化脂肪酸的57钴肥皂分析法与气液色谱法的比较。
A 57Co-soap assay for plasma total non-esterified fatty acids compared with a gas-liquid chromatographic method.
作者信息
Turnell D C, Price C P, France M M
出版信息
Clin Chem. 1980 Dec;26(13):1879-82.
PMID:7438436
Abstract
We describe a relatively rapid radiochemical assay for determining plasma non-esterified fatty acids, by use of a Dole-type (J. Clin. Invest. 35: 150, 1956) extraction and formation of 57Co soaps. Results correlated well (r = 0.957) with those by gas-liquid chromatography. The between-batch CV for 1.12 mmol of non-esterified fatty acids per liter was 7%; the sensitivity is estimated to be 22 mumol/L.
摘要
我们描述了一种相对快速的放射化学分析方法，用于测定血浆中非酯化脂肪酸，该方法采用Dole型（《临床研究杂志》35: 150, 1956）萃取法并形成57Co肥皂。结果与气相色谱法的结果相关性良好（r = 0.957）。每升含1.12 mmol非酯化脂肪酸时，批间变异系数为7%；灵敏度估计为22 μmol/L。
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