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与公羊体细胞线粒体DNA相比,精子线粒体DNA的独特性。

Uniqueness of sperm mtDNA as compared to somatic mtDNA in ram.

作者信息

Bartoov B, Fisher J

出版信息

Int J Androl. 1980 Oct;3(5):594-601. doi: 10.1111/j.1365-2605.1980.tb00147.x.

Abstract

Sperm mtDNA exhibits unique physicochemical properties compared with mtDNA from somatic tissues in the ram. The buoyant density of sperm mtDNA was 1,6983 g/cm3 while the brain, heart and liver mtDNAs was about 1.7005 g/cm3. The Tm of the liver and sperm mtDNA was 71.0 degrees C and 69.5 degrees C, respectively. The G + C content of sperm mtDNA was 3.0 moles % lower than the liver mtDNA. The contour length of the circular sperm mtDNA was 5.01 micrometers compared with the liver mtDNA with contour length of 5.33 micrometers.

摘要

与公羊体细胞组织中的线粒体DNA(mtDNA)相比,精子mtDNA表现出独特的物理化学性质。精子mtDNA的浮力密度为1.6983 g/cm³,而脑、心脏和肝脏的mtDNA浮力密度约为1.7005 g/cm³。肝脏和精子mtDNA的解链温度分别为71.0℃和69.5℃。精子mtDNA的鸟嘌呤与胞嘧啶(G + C)含量比肝脏mtDNA低3.0摩尔%。环形精子mtDNA的轮廓长度为5.01微米,而肝脏mtDNA的轮廓长度为5.33微米。

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