Histofluorescence technique for the intracellular localization of chemical carcinogens.

Many carcinogens exhibit a fluorescent emission when excited with ultraviolet light. Advantage has been taken of this property to develop a technique that can detect microquantities of these carcinogens on conventional microscopic tissue preparations. This work describes the localization of aflatoxin B1, N-2-fluorenylacetamide and benzo(a)pyrene both in the cell cytoplasm and nucleus after in vivo administration of these compounds.