Satyaswaroop P G, Gurpide E
Endocrinology. 1978 Jun;102(6):1761-5. doi: 10.1210/endo-102-6-1761.
Sixty-day-old rats were divided into four groups and treated for 30 days with either medroxyprogesterone acetate (Provera), gonadotropins (bovine LH and ovine FSH), Provera plus gonadotropins, or saline. The progestin treatment resulted in a lowering of plasma levels of testosterone, androstenedione, and LH, as well as in a reduction of epididymal sperm counts and accessory sex organ weights. The progestin-treated groups showed markedly lower levels of testicular 17 beta-hydroxysteroid dehydrogenase activity (35% of controls) and delta 5,3 beta-hydroxysteroid dehydrogenase activity (70% of controls). Rats treated with only gonadotropins exhibited reduced 17 beta-hydroxysteroid dehydrogenase but increased delta 5,3 beta-hydroxysteroid dehydrogenase activities. It was concluded from these results that progestins may affect testicular steroidogenesis and spermatogenesis not only by reducing LH secretion but also by a direct effect on the testis, as LH suppression could not account for the inhibition of 17 beta-hydroxysteroid dehydrogenase activity. Long term progestin treatment did not alter the steroidogenic response of the testis to acute administration of LH, although the testosterone to androstenedione ratio in plasma was decreased.
60日龄大鼠被分为四组，分别用醋酸甲羟孕酮（安宫黄体酮）、促性腺激素（牛促黄体素和羊促卵泡素）、醋酸甲羟孕酮加促性腺激素或生理盐水处理30天。孕激素处理导致血浆睾酮、雄烯二酮和促黄体素水平降低，同时附睾精子计数和附属生殖器官重量减少。经孕激素处理的组睾丸17β-羟基类固醇脱氢酶活性（为对照组的35%）和δ5,3β-羟基类固醇脱氢酶活性（为对照组的70%）明显较低。仅用促性腺激素处理的大鼠17β-羟基类固醇脱氢酶活性降低，但δ5,3β-羟基类固醇脱氢酶活性增加。从这些结果得出的结论是，孕激素可能不仅通过减少促黄体素分泌，而且通过对睾丸的直接作用来影响睾丸类固醇生成和精子发生，因为促黄体素抑制不能解释17β-羟基类固醇脱氢酶活性的抑制。长期孕激素处理并未改变睾丸对急性给予促黄体素的类固醇生成反应，尽管血浆中睾酮与雄烯二酮的比率降低。
