Schleich H G, Wiest W
J Cancer Res Clin Oncol. 1980;97(3):307-14. doi: 10.1007/BF00405783.
The levels of total human serum RNAase activity (HSRA) from 24 patients with ovarian cancer have been followed up for a period of over 5 months. The determinations of HSRA were carried out according to our assay system described earlier. Two representative patterns of the HSRA are presented, one from a patient with an ascertained remission of an ovarian carcinoma. Increasing HSRA values are concomitant with tumor progression while in remission the HSRA remains at normal rates. We have reported earlier that optimum HSRA depends on physiological concentrations of NaCl in the reaction mixture. However, NaCl can be replaced by KCl without a loss of activity. No correlation was detectable between increased HSRA and an excessive electrolyte content in serum. The mixtures of sera with various RNAase activities showed linear additivity. These findings suggest that an increased concentration of the RNAases normally present in human serum and/or additional RNAases are responsible for the increase of the HSRA.
对24例卵巢癌患者的人血清总RNA酶活性（HSRA）水平进行了5个多月的随访。HSRA的测定是根据我们先前描述的检测系统进行的。给出了两种具有代表性的HSRA模式，一种来自一名确诊为卵巢癌缓解期的患者。HSRA值的增加与肿瘤进展相伴，而在缓解期HSRA保持在正常水平。我们先前曾报道，最佳HSRA取决于反应混合物中NaCl的生理浓度。然而，KCl可替代NaCl而不损失活性。HSRA升高与血清中电解质含量过高之间未检测到相关性。具有不同RNA酶活性的血清混合物表现出线性加和性。这些发现表明，人血清中正常存在的RNA酶浓度增加和/或额外的RNA酶是HSRA升高的原因。
