Moore T J, Braley L M, Williams G H
Endocrinology. 1978 Jul;103(1):152-5. doi: 10.1210/endo-103-1-152.
The control of 18-hydroxy-11-deoxycorticosterone (18-OH-DOC) secretion is incompletely understood: ACTH seems to be the dominant regulator, the importance of angiotensin II (A-II) is uncertain, and the effect of potassium has not been investigated. The purpose of this study was to evaluate the 18-OH-DOC response to these three stimuli in vitro. Suspensions of isolated rat adrenal glomerulosa or fasciculata cells were stimulated with either alpha-1-24 ACTH (0.04 mU/ml), A-II (25 ng/ml), or potassium (5.9 mEq/liter), and 18-OH-DOC production was measured. In glomerulosa cells, ACTH produced the greatest 18-OH-DOC response but A-II and potassium also produced significant (P less than 0.001) 18-OH-DOC increases (control, 162 +/- 14 (SE) ng/10(6) cells incubated; A-II, 368 +/- 39; potassium, 380 +/- 37; ACTH, 1544 +/- 165). In fasciculata cells, 18-OH-DOC production increased with ACTH but not with A-II or potassium. These results document that A-II and potassium, as well as ACTH, can stimulate 18-OH-DOC production by glomerulosa but not by fascicuata cells. The response to A-II may provide an explanation for the reported increase in 18-OH-DOC production after sodium restriction.
18-羟-11-脱氧皮质酮（18-OH-DOC）分泌的调控机制尚未完全明确：促肾上腺皮质激素（ACTH）似乎是主要调节因子，血管紧张素II（A-II）的作用尚不明确，钾的影响也未得到研究。本研究旨在评估体外条件下这三种刺激对18-OH-DOC的反应。分离的大鼠肾上腺球状带或束状带细胞悬液分别用α-1-24 ACTH（0.04 mU/ml）、A-II（25 ng/ml）或钾（5.9 mEq/升）刺激，然后测定18-OH-DOC的生成量。在球状带细胞中，ACTH引起的18-OH-DOC反应最大，但A-II和钾也能显著（P<0.001）增加18-OH-DOC的生成量（对照组，每10⁶个孵育细胞中为162±14（SE）ng；A-II组，368±39；钾组，380±37；ACTH组，1544±165）。在束状带细胞中，18-OH-DOC的生成量随ACTH增加，但不随A-II或钾增加。这些结果表明，A-II、钾以及ACTH均可刺激球状带细胞生成18-OH-DOC，但不能刺激束状带细胞。对A-II的反应可能解释了钠限制后报道的18-OH-DOC生成量增加的现象。
