High-performance liquid chromatography and thin-layer chromatography of anthracycline antibiotics: separation and identification of components of the dauno-rubicin complex from fermentation broth.

A new solvent system composed of methanol-acidic water (pH 2.0 with phosphoric acid) has been developed for high-performance liquid chromatography of the daunorubicin complex from fermentation broth on a micro Bondapak C18 column. Application of this solvent system in conjunction with thin-layer chromatography in the identification of anthracycline antibiotics from the fermentation broth is discussed.