Sananès N, Weiller S, Baulieu E E, Le Goascogne C
Endocrinology. 1978 Jul;103(1):86-95. doi: 10.1210/endo-103-1-86.
Rat endometrial cells were isolated from ovariectomized adults submitted to a progesterone-estradiol sequence proper to sensitize the uterus to respond to a deciduogenic stimulus, and then cultured in vitro in the presence of progesterone. They grew rapidly and became morphologically similar to the decidual cells occurring in vivo with regard to the binucleation and the accumulation of characteristic filamentous material in the cytoplasm. The life-span of the decidualized cells in vitro was comparable to the duration of the deciduoma in vivo. The progesterone treatment of the rat was found to be a prerequisite for such development and transformation of endometrial cells in vitro, since control experiments performed with nonhormonally injected castrated animals gave only isolated islets of epithelial cells and scattered fibroblast-like cells in culture, whereas the decidual transformation was still observed, although with some delay, with cells from progesterone (but not estradiol)-treated spayed rats. Such an in vitro system may provide a new opportunity to study the mechanism of decidualization and of its hormonal control.
从接受适当孕酮 - 雌二醇序列处理以使子宫对蜕膜化刺激产生反应的成年去卵巢大鼠中分离出子宫内膜细胞，然后在孕酮存在的情况下进行体外培养。它们生长迅速，在双核化以及细胞质中特征性丝状物质的积累方面，形态上变得与体内出现的蜕膜细胞相似。体外蜕膜化细胞的寿命与体内蜕膜瘤的持续时间相当。发现对大鼠进行孕酮处理是体外子宫内膜细胞发生这种发育和转化的先决条件，因为对未接受激素注射的阉割动物进行的对照实验在培养物中仅产生孤立的上皮细胞岛和散在的成纤维细胞样细胞，而用孕酮（而非雌二醇）处理的去势大鼠的细胞，尽管有一些延迟，但仍观察到蜕膜化转变。这样的体外系统可能为研究蜕膜化及其激素控制机制提供新的机会。
