West D P, Fillenz M
J Neurochem. 1980 Dec;35(6):1323-8. doi: 10.1111/j.1471-4159.1980.tb09005.x.
The noradrenaline storage capacity of vesicles in hypothalamic synaptosomes was measured by incubating them with [3H]noradrenaline under saturating conditions. The normal noradrenaline content is 52% of storage capacity. Incubation or superfusion with 50 mM-potassium causes calsium-dependent release from the vesicles. Such release reduces not only the vesicular content, but also the noradrenaline storage capacity. This suggests that after exocytosis vesicles cannot refill with noradrenaline.
