Kovachich G B, Mishra O P
J Neurochem. 1980 Dec;35(6):1449-52. doi: 10.1111/j.1471-4159.1980.tb09022.x.
Malonaldehyde formation by cortical brain slices from rat brain was determined as a function of incubation time and of oxygen pressure. This substance, a byproduce of lipid peroxidation, was detected by the thiobarbituric acid test. Significant amounts of malonaldehyde were formed by brain slices during incubation in the 0.2 (air) to 10 atm oxygen range, and a portion of it was released into the medium. The rate of malonaldehyde formation was the highest during the first 10 min. Elevation of oxygen pressure above 1 atm caused further increments in malonaldehyde production with kinetic properties similar to that seen at 1 atm pressure, but the increments per additional oxygen pressure were diminishing. The formation of a given amount of malonaldehyde can be expressed as a function of atm oxygen X min. This function has the shape of a saturation curve approaching a maximum at around 300 atm X min. The results indicate extensive lipid peroxidation in brain slices under standard incubation conditions.
测定了大鼠大脑皮质脑片丙二醛的生成量与孵育时间和氧分压的关系。这种脂质过氧化的副产物通过硫代巴比妥酸试验检测。在0.2（空气）至10个大气压的氧范围内孵育期间，脑片形成了大量丙二醛，其中一部分释放到培养基中。丙二醛生成速率在最初10分钟内最高。氧分压升高至1个大气压以上会导致丙二醛生成进一步增加，其动力学特性与1个大气压时相似，但每增加一个氧分压的增量在减小。一定量丙二醛的生成可以表示为大气压氧×分钟的函数。该函数呈饱和曲线形状，在约300大气压×分钟时接近最大值。结果表明在标准孵育条件下脑片存在广泛的脂质过氧化。
