Corwin L M, Shloss J
J Nutr. 1980 Dec;110(12):2497-505. doi: 10.1093/jn/110.12.2497.
By comparing the activities of tocopherol with other antioxidants and tocopherol analogs on mitogenesis of murine spleen cells, an attempt was made to understand the mechanism of tocopherol stimulation. N,N'-diphenyl-p-phenylene diamine (DPPD) was very active in this system, whereas butylated hydroxytoluene, another antioxidant, was much less active. Trolox C, an analog of alpha-tocopherol with a carboxyl group instead of the isoprene side chain was totally inactive. Tocopherol quinone, an oxidized form of tocopherol, was almost as active as tocopherol. Menadione (vitamin K-3), a quinone without a side chain, was also active. It was suggested therefore, that although in tocopherol-like compounds the side chain seems important, the activities of DPPD and menadione clearly do not require it. On the other hand, the stimulation by tocopherol quinone and menadione appears to rule out a requirement for an antioxidant function in mitogenic stimulation. A comparison of the action of tocopherol with indomethacin ruled out regulation of the peroxidation step leading to prostaglandin synthesis as the mechanism for the tocopherol effect.
通过比较生育酚与其他抗氧化剂以及生育酚类似物对小鼠脾细胞有丝分裂的活性，试图了解生育酚刺激的机制。N，N'-二苯基对苯二胺（DPPD）在该系统中非常活跃，而另一种抗氧化剂丁基化羟基甲苯的活性则低得多。生育三烯酚C是α-生育酚的类似物，具有羧基而非异戊二烯侧链，完全没有活性。生育酚醌是生育酚的氧化形式，其活性几乎与生育酚相同。甲萘醌（维生素K-3）是一种没有侧链的醌，也具有活性。因此有人提出，虽然在生育酚样化合物中侧链似乎很重要，但DPPD和甲萘醌的活性显然不需要它。另一方面，生育酚醌和甲萘醌的刺激似乎排除了有丝分裂刺激中对抗氧化功能的需求。将生育酚与吲哚美辛的作用进行比较，排除了导致前列腺素合成的过氧化步骤的调节作为生育酚效应的机制。
