Grounds M, Partridge T A, Sloper J C
J Pathol. 1980 Dec;132(4):325-41. doi: 10.1002/path.1711320404.
A sequential study of 180 allografts of minced skeletal muscle has been made in mice, in 124 of which tolerance was induced. The host/donor composition of grafts was assessed in terms of their content of isoenzymes of glucose-6-phosphate isomerase characteristic of host and donor strains. From 0-5 days implanted muscle fibre fragments uniformly underwent degeneration. New myotubes appeared at day 5. Both host and donor isoenzyme were found during this period. In "non-tolerant" hosts, grafts were rejected at 8-12 days, after which only host isoenzyme was found and the graft site usually lacked muscle, consisting of fibrofatty connective tissue. In the few instances where muscle was found in such grafts, this was necessarily formed from host precursor cells which had migrated into the graft site. In "tolerant" hosts, grafts contained up to 80 per cent. of the muscle and usually yielded both host and donor isoenzyme. Where "hybrid" isoenzyme was found, it was probable that host muscle precursor-cells had entered grafts and fused with donor muscle.
对小鼠体内180块切碎的骨骼肌同种异体移植进行了一项序贯研究，其中124例诱导出了耐受性。根据移植组织中宿主和供体菌株特有的葡萄糖-6-磷酸异构酶同工酶含量，评估移植组织的宿主/供体组成。植入后0至5天，肌纤维片段均发生变性。第5天出现新的肌管。在此期间发现了宿主和供体同工酶。在“非耐受性”宿主中，移植组织在8至12天被排斥，之后仅发现宿主同工酶，移植部位通常没有肌肉，由纤维脂肪结缔组织组成。在少数此类移植组织中发现有肌肉的情况下，这必然是由迁移到移植部位的宿主前体细胞形成的。在“耐受性”宿主中，移植组织含有高达80%的肌肉，通常同时产生宿主和供体同工酶。当发现“杂交”同工酶时，很可能宿主肌肉前体细胞已进入移植组织并与供体肌肉融合。
