Watt D J, Morgan J E, Partridge T A
Department of Anatomy, Charing Cross and Westminster Medical School, London, U.K.
Neuromuscul Disord. 1991;1(5):345-55. doi: 10.1016/0960-8966(91)90121-8.
Implantation of normal muscle precursor cells into myopathic fibres to alleviate recessively inherited diseases of skeletal muscle has received much attention since the discovery of a defective or deficient gene coding for the protein dystrophin in the Duchenne and Becker forms of muscular dystrophy. Therapeutic allografting of cells would require some means of preventing their immune rejection. Here we have allografted muscle into the non-tolerant and non-immunosuppressed murine host. Precursor cells introduced in the form of a single cell suspension survive for prolonged periods post-implantation. Allografts of minced muscle often failed to survive, even though host and donor were compatible at the major histocompatibility locus. Differences at minor loci may well have contributed to such rejection. Where allografted tissue was rejected, there was a decrease in the amount of surviving host muscle at the graft site, an important observation in terms of the therapeutic implantation of cells.
自从在杜兴氏和贝克氏型肌营养不良症中发现编码抗肌萎缩蛋白的基因存在缺陷或不足以来，将正常肌肉前体细胞植入肌病纤维以缓解隐性遗传性骨骼肌疾病受到了广泛关注。细胞治疗性同种异体移植需要某种方法来防止免疫排斥。在此，我们将肌肉同种异体移植到未经耐受和未免疫抑制的小鼠宿主中。以单细胞悬液形式引入的前体细胞在植入后能长期存活。切碎肌肉的同种异体移植往往无法存活，即使宿主和供体在主要组织相容性位点相匹配。次要位点的差异很可能导致了这种排斥反应。当同种异体移植组织被排斥时，移植部位存活的宿主肌肉量会减少，这对于细胞治疗性植入而言是一项重要的观察结果。
