Watt D J, Morgan J E, Partridge T A
Clin Exp Immunol. 1984 Feb;55(2):419-26.
Previous work (Watt et al., 1982) has shown that, in the mouse, skeletal muscle can be transplanted successfully in the form of a suspension of it's mononucleate precursors. Eventual therapeutic application of this technique by the implantation of precursor cells derived from normal muscle into myopathic individuals would require a means of preventing allograft rejection applicable to man. We have therefore investigated the use of the drug cyclosporin A (CyA) as a means of prolonging the survival in mice of allografts of mononucleate muscle cells made into a region of regenerating host muscle. We have administered CyA to the hosts at doses of either 75 or 150 mg/kg body weight/day for 42 days from the day of grafting. By using isoenzyme allotypes as markers of host and donor tissues, we have shown that allografted mononucleate cells become incorporated in host muscle fibres and that the mosaic host/donor muscle fibres so formed survive for as long as we continued the experiment, a maximum of 107 days after grafting, or 65 days after the end of CyA treatment.
先前的研究工作（瓦特等人，1982年）表明，在小鼠中，骨骼肌可以以其单核前体细胞悬液的形式成功移植。最终，通过将源自正常肌肉的前体细胞植入患有肌病的个体来应用这项技术，将需要一种适用于人类的防止同种异体移植排斥的方法。因此，我们研究了使用药物环孢菌素A（CyA）作为延长单核肌肉细胞同种异体移植在小鼠体内存活时间的一种手段，该移植是在宿主肌肉再生区域进行的。从移植当天起，我们以75或150毫克/千克体重/天的剂量给宿主施用CyA，持续42天。通过使用同工酶同种异型作为宿主和供体组织的标记，我们已经表明，同种异体移植的单核细胞融入了宿主肌肉纤维中，并且如此形成的嵌合宿主/供体肌肉纤维在我们持续实验的时间内一直存活，移植后最长存活107天，或在CyA治疗结束后65天。
