Heck H A, Kluwe C L
J Anal Toxicol. 1980 Sep-Oct;4(5):222-6. doi: 10.1093/jat/4.5.222.
Quantitative methods are described for the analysis of pH, sodium, ammonium, potassium, calcium, magnesium, chloride, phosphate, and sulfate, as well as terephthalic acid and dimethyl terephthalate, in a single urine sample as small as 20 microliter. The procedure utilizes ion chromatography and atomic absorption for electrolyte analysis, a microelectrode for pH measurement, and high-performance liquid chromatography for analysis of the organic compounds. The techniques are applied to urine samples freshly collected from rats ingesting dietary dimethyl terephthalate. Specific changes in urinary ions, including hypercalciuria and urinary acidosis, are shown to develop as a consequence of dimethyl terephthalate ingestion. The results indicate that metabolism of dimethyl terephthalate to terephthalic acid occurs extensively in Fischer-344 rats, and accounts for the ion changes that are observed.
本文描述了定量分析方法，可对仅20微升的单一尿液样本中的pH值、钠、铵、钾、钙、镁、氯、磷酸盐和硫酸盐以及对苯二甲酸和对苯二甲酸二甲酯进行分析。该程序利用离子色谱法和原子吸收法进行电解质分析，使用微电极测量pH值，并采用高效液相色谱法分析有机化合物。这些技术应用于从摄入对苯二甲酸二甲酯饮食的大鼠新鲜采集的尿液样本。结果显示，摄入对苯二甲酸二甲酯会导致尿离子发生特定变化，包括高钙尿症和尿酸性中毒。结果表明，在Fischer-344大鼠中，对苯二甲酸二甲酯广泛代谢为对苯二甲酸，这解释了所观察到的离子变化。
