Illingworth D R, Naylor I L
J Pharmacol Methods. 1980 Nov;4(3):197-207. doi: 10.1016/0160-5402(80)90011-x.
The development and use of an electronic integrator suitable for use with isolated tissues is described. The design of the apparatus enables a multichannel integrator to be built from easily obtainable components at a relatively low cost while having accuracy and stability not previously available from circuits designed for such applications. The design includes a Schmitt trigger for positive reset of the integrator section, a chopper type linear amplifier, an electronic digital totalizing counter with seen segment LED readout, and the inclusion of an FET device as the discharge element. The addition of a polarity change switch accommodates both relaxatory and contractile responses. A switched attenuator enables 0-10 mV, 0-100 mV, 0-1 V, and 0-14 V ranges of input to be covered. The integrator enables accurate quantitation of contractile and relaxatory responses and also allows myogenic activity to be measured whatever the time course of these responses. This integrator thereby provides an accurate alternative to the usual quantitative measurements determined from isolated tissues.
本文描述了一种适用于离体组织的电子积分器的研制与应用。该仪器的设计使得多通道积分器能够用容易获得的组件以相对较低的成本构建而成，同时具备此前此类应用电路所没有的精度和稳定性。其设计包括用于积分器部分正向复位的施密特触发器、斩波型线性放大器、带有七段LED读数的电子数字累加计数器，以及采用场效应晶体管（FET）器件作为放电元件。增设极性转换开关可适应舒张和收缩反应。开关衰减器能够覆盖0 - 10 mV、0 - 100 mV、0 - 1 V和0 - 14 V的输入范围。该积分器能够准确量化收缩和舒张反应，并且无论这些反应的时间进程如何，都能测量肌源性活动。因此，这种积分器为从离体组织进行的常规定量测量提供了一种准确的替代方法。
