Rosenthal M D
Lipids. 1980 Oct;15(10):838-48. doi: 10.1007/BF02534374.
Fetal human fibroblasts were grown in culture medium containing 10% fetal bovine serum supplemented with [1(-14)C]linoleate or [1(-14)C]oleate. At all concentrations of exogenous fatty acids, the incorporation of oleate was greater than that of linoleate. With increased medium fatty acid concentrations, linoleate in triacyglycerol (TAG) could be increased from 13 to 75% of the total incorporated; at each concentration, relatively more linoleate than oleate was in TAG. When the cells were exposed to exogenous oleate/linoleate mixtures, the composition of the mixture determined the extent of incorporation of both fatty acids. When the mixture was primarily linoleate, scarce oleate was used preferentially for phospholipids (PL); no such specificity for scarce linoleate was observed. Addition of exogenous fatty acids resulted in a shift of previously incorporated 14C fatty acids from phospholipid into TAG; retention of oleate in PL was greater than that of linoleate. Incorporation of oleate into phospholipids was also higher than that of linoleate from exogenous fatty acid mixtures which were 80% saturated. It is suggested that normal human fibroblasts have adapted to the low levels of exogenous polyunsaturated fatty acids in culture media by increased use of oleate in phospholipid. Even when the cells are supplemented with linoleate, the preferential use of oleate in phospholipid groups is retained.
将人胎儿成纤维细胞培养于含有10%胎牛血清并补充了[1(-14)C]亚油酸或[1(-14)C]油酸的培养基中。在所有外源性脂肪酸浓度下，油酸的掺入量均高于亚油酸。随着培养基脂肪酸浓度的增加，三酰甘油（TAG）中亚油酸的含量可从总掺入量的13%增加至75%；在每个浓度下，TAG中亚油酸的含量相对高于油酸。当细胞暴露于外源性油酸/亚油酸混合物时，混合物的组成决定了两种脂肪酸的掺入程度。当混合物主要为亚油酸时，稀少的油酸优先用于磷脂（PL）的合成；未观察到对稀少亚油酸有此类特异性。添加外源性脂肪酸导致先前掺入的14C脂肪酸从磷脂转移至TAG；油酸在PL中的保留量高于亚油酸。从80%饱和的外源性脂肪酸混合物中，油酸掺入磷脂的量也高于亚油酸。提示正常人成纤维细胞通过增加在磷脂中使用油酸来适应培养基中低水平的外源性多不饱和脂肪酸。即使细胞补充了亚油酸，在磷脂基团中优先使用油酸的情况仍会保留。
