Robert J, Rebel G, Mandel P
Biochimie. 1977;59(4):417-23. doi: 10.1016/s0300-9084(77)80318-0.
The fetal calf serum on which two astroblast cell lines were grown was shown to be deficient in essential fatty acids. The fatty acid profiles of lipids of these two cell lines showed very low amounts of polyunsaturated fatty acids. In order to know if this low level of unsaturation was due to the lack of essential fatty acids in serum, or to a lack of desaturase activities in the cells, we have investigated the modifications of the cell lipid fatty acid patterns when serum was enriched in essential fatty acids. Linoleic acid was incorporated in rather high amounts in the cell lipids, while linolenic acid was very poorly incorporated. These two essential fatty acids were converted into polyunsaturated fatty acids only when they were added alone to the serum. Both cell clones exhibited a lack in the delta 4 desaturase activity. No morphological changes of the cells occured after nine days of culture with modified serum.
在其上培养两种成星形细胞系的胎牛血清被证明缺乏必需脂肪酸。这两种细胞系的脂质脂肪酸谱显示多不饱和脂肪酸含量非常低。为了弄清楚这种低不饱和水平是由于血清中缺乏必需脂肪酸，还是由于细胞中缺乏去饱和酶活性，我们研究了在血清中富含必需脂肪酸时细胞脂质脂肪酸模式的变化。亚油酸大量掺入细胞脂质中，而亚麻酸掺入量非常少。只有当这两种必需脂肪酸单独添加到血清中时，它们才会转化为多不饱和脂肪酸。两个细胞克隆均表现出缺乏δ4去饱和酶活性。用改良血清培养九天后，细胞未出现形态变化。
