Spector A A, Denning G M, Stoll L L
In Vitro. 1980 Nov;16(11):932-40. doi: 10.1007/BF02619331.
The fatty acid composition of cultured human skin fibroblasts was modified by adding either oleic or linoleic acid to the growth medium. After the cultures became confluent, they were washed and transferred to different maintenance media in order to determine the stability of the various fatty acyl modifications. Some changes in fatty acid composition occurred under all conditions. When the maintenance medium was supplemented with fatty acid, the cellular neutral lipid and phospholipid fatty acyl composition were altered markedly within 16 to 24 hr. If no supplemental fatty acid was available during the maintenance period, however, the modified fatty acyl compositions were sufficiently retained so that appreciable differences between the cells enriched with oleate and linoleate persisted for at least 48 to 72 hr. This considerable degree of stability occurred when either 10% delipidized fetal bovine serum or 10% fetal bovine serum containing its inherent lipids were present in the maintenance medium. Although the triglyceride content of the fatty acid-modified cells was quite labile, neither the cholesterol nor phospholipid content changed appreciably during culture in any of the maintenance media. Since the fatty acid compositional differences persisted during several days of maintenance under certain conditions, these modified cultures appear to be a useful experimental system for assessing the effect of lipid structure on fairly long-term cellular functions.
通过向生长培养基中添加油酸或亚油酸，可改变培养的人皮肤成纤维细胞的脂肪酸组成。当培养物汇合后，将其洗涤并转移至不同的维持培养基中，以确定各种脂肪酸酰基修饰的稳定性。在所有条件下，脂肪酸组成均发生了一些变化。当维持培养基中添加脂肪酸时，细胞中性脂质和磷脂的脂肪酸酰基组成在16至24小时内会发生显著改变。然而，如果在维持期内没有补充脂肪酸，修饰后的脂肪酸酰基组成会得到充分保留，以至于富含油酸酯和亚油酸酯的细胞之间的明显差异至少持续48至72小时。当维持培养基中存在10%脱脂胎牛血清或含有其固有脂质的10%胎牛血清时，就会出现这种相当程度的稳定性。尽管脂肪酸修饰细胞的甘油三酯含量相当不稳定，但在任何维持培养基中培养期间，胆固醇和磷脂含量均未发生明显变化。由于在某些条件下，脂肪酸组成差异在维持数天期间持续存在，这些修饰后的培养物似乎是评估脂质结构对相当长期细胞功能影响的有用实验系统。
