Bykovskaia S V, Trotsenko Iu A
Mikrobiologiia. 1980 Sep-Oct;49(5):695-701.
The activity of the following enzymes was investigated in cell extracts of Candida methylica cultivated in media with methanol or glucose: the enzymes of glycolysis, the pentose phosphate pathway, the citric acid cycle and the glyoxylate shunt; pyruvate and phosphoenolpyruvate carboxylases; dehydrogenases of amino acids. Different activities of the enzymes suggest that metabolic pathways for these substrates are alternative and that methanol is assimilated by the yeast via the dihydroxyacetone pathway. A scheme for methanol metabolism in Candida methylica is proposed and discussed.
在以甲醇或葡萄糖为培养基培养的甲基假丝酵母细胞提取物中，研究了以下几种酶的活性：糖酵解酶、磷酸戊糖途径酶、柠檬酸循环酶和乙醛酸循环支路酶；丙酮酸羧化酶和磷酸烯醇丙酮酸羧化酶；氨基酸脱氢酶。这些酶的不同活性表明，这些底物的代谢途径是交替的，并且甲醇是通过二羟基丙酮途径被酵母同化的。本文提出并讨论了甲基假丝酵母中甲醇代谢的方案。
