Kalacheva G S, Volova T G, Trubachev I N
Mikrobiologiia. 1980 Sep-Oct;49(5):727-33.
The composition of lipids was studied in three strains of carboxydobacteria, viz. Seliberia carboxydohydrogena Z-1062, Pseudomonas gazotropha Z-1156 and Comamonas compransoris Z-1155. The content of lipids extracted with an alcohol--chloroform mixture varied in these strains from 9 to 13.2%. The main classes were phospholipids (from 62.6 to 77.2% of the overall lipid content). The major phospholipids were phosphatidyl ethanolamine and phosphatidyl choline. Moreover, the strains Z-1062 and Z-1156 contained three unidentified phospholipids which constituted 40 and 20% of the total content respectively. The neutral lipids of the carboxydobacteria comprised diglycerides, triglycerides, free fatty acids, methyl esters of fatty acids and a number of compounds which were not identified. The fatty acids of the strains were represented by saturated, monoene and cyclopropanoic acids. The main acids were palmitic, palmitolenic, C17-cyclopropanoic, stearic and octadecenic acids. Polar lipids differed from neutral lipids in their fatty acid composition. The fatty acid composition of firmly bound lipids was characterized by a high degree of saturation, the absence of cyclopropanoic acids and the presence of two unidentified acids that were not found in free lipids; apparently, they belonged to hydroxy acids.
对三株一氧化碳细菌的脂质组成进行了研究,即羧基氢单胞菌Z-1062、嗜气假单胞菌Z-1156和综合贪铜菌Z-1155。用乙醇 - 氯仿混合物提取的脂质含量在这些菌株中从9%到13.2%不等。主要类别是磷脂(占总脂质含量的62.6%至77.2%)。主要的磷脂是磷脂酰乙醇胺和磷脂酰胆碱。此外,Z-1062和Z-1156菌株含有三种未鉴定的磷脂,分别占总含量的40%和20%。一氧化碳细菌的中性脂质包括甘油二酯、甘油三酯、游离脂肪酸、脂肪酸甲酯和一些未鉴定的化合物。这些菌株的脂肪酸由饱和脂肪酸、单烯酸和环丙烷酸组成。主要的酸是棕榈酸、棕榈油酸、C17 - 环丙烷酸、硬脂酸和十八碳烯酸。极性脂质在脂肪酸组成上与中性脂质不同。紧密结合脂质的脂肪酸组成的特点是饱和度高、不存在环丙烷酸以及存在两种在游离脂质中未发现的未鉴定酸;显然,它们属于羟基酸。