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14C 标记的甘油三油酸酯在大豆油乳剂中的组织分布。大鼠和小鼠的放射自显影研究。

Tissue distribution of 14C-labelled triolein in a soybean oil emulsion. An autoradiographic study in rat and mouse.

作者信息

Appelgren L E, Rössner S

出版信息

Nutr Metab. 1980;24(2):122-34. doi: 10.1159/000176332.

DOI:10.1159/000176332
PMID:7443090
Abstract

A fed and a starved group of mice were injected with a 14C-labelled triolein emulsion, similar to Intralipid, in a dose corresponding to 0.1 mg triglyceride per gram body weight. The animals were sacrificed from 30 sec to 24 h after injection. The tissue distribution of the fat emulsion depended on the nutritional state of the animals. A marked concentration of radioactivity was found in the gastric mucosa of all animals as soon as 30 sec after injection which declined with time but was still clearly discernible in the autoradiograms 24 h after injection. Although the radioactivity only demonstrates the presence of the labelled oleic acid without indication of the degree of triglyceride lipolysis, the autoradiograms showed marked differences in the concentration of radioactivity between the various groups of skeletal muscles and between the different parts of the reticulo-endothelial system.

摘要

给一组喂食的小鼠和一组饥饿的小鼠注射了一种与英脱利匹特类似的、14C标记的三油酸甘油酯乳剂,剂量相当于每克体重0.1毫克甘油三酯。在注射后30秒至24小时处死动物。脂肪乳剂的组织分布取决于动物的营养状态。注射后30秒,在所有动物的胃黏膜中就发现了明显的放射性聚集,其随时间下降,但在注射后24小时的放射自显影片中仍清晰可辨。虽然放射性仅表明标记油酸的存在,而未显示甘油三酯脂解程度,但放射自显影片显示,不同组的骨骼肌之间以及网状内皮系统的不同部位之间,放射性浓度存在显著差异。

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