Howard R J, Smith P M, Mitchell G F
Parasitology. 1980 Oct;81(2):299-314. doi: 10.1017/s0031182000056043.
Lactoperoxidase-catalysed radio-iodination was used to compare the surface proteins on red cells from Plasmodium yoelii-infected with normal BALB/c mice. The profile of radio-iodinated proteins separated by SDS-polyacrylamide gel electrophoresis was different for infected blood of similar parasitaemia from mice inoculated with different doses of the parasite. Inoculation with different doses of the parasite. Inoculation with the lower dose resulted in the appearance of a major radio-iodinated protein of apparent molecular weight (Mr) 76 000 which was labelled to a similar extent on uninfected red cells from infected blood and purified multinucleate infected cells. Several minor radio-iodinated bands, with identical mobilities to the minor bands on normal BALB/c erythrocytes, were also present on red cells from this infected blood. In contrast, the higher inoculation dose produced changes in the minor labelled bands, and the band with Mr of 76 000 was absent. In this case, the minor radio-iodinated proteins of the normal BALB/c erythrocyte (with Mr of 65 000, 57 000, 48 000, 38 000 and 32 000) were replaced by a series of bands with Mr of 60 000, 50 000, 43 000 and 28 000 on both uninfected and infected red cells. These differences with inoculation dose may be related to the different duration of these infections, the development of anaemia and the extent of pathological changes at the erythrocyte surface. P. yoelii infection caused a marked loss in periodate-dependent labelling of sialoglycoproteins on most, if not all, red cells in infected blood. There was also a large decrease in galactose oxidase-dependent glycoprotein labelling with or without neuraminidase treatment. These changes in the carbohydrate groups on red cell membrane glycoproteins may be linked to the excessive loss of both uninfected and infected red cells during some malaria infections.
利用乳过氧化物酶催化的放射性碘化作用，比较约氏疟原虫感染的小鼠与正常BALB/c小鼠红细胞表面的蛋白质。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离的放射性碘化蛋白质图谱，对于接种不同剂量疟原虫的小鼠中具有相似疟原虫血症的感染血液来说是不同的。接种不同剂量的疟原虫。接种较低剂量会导致出现一种表观分子量（Mr）为76000的主要放射性碘化蛋白质，在来自感染血液的未感染红细胞和纯化的多核感染细胞上，其标记程度相似。在这种感染血液的红细胞上，还存在几条次要的放射性碘化条带，其迁移率与正常BALB/c红细胞上的次要条带相同。相比之下，较高的接种剂量使次要标记条带发生了变化，且表观分子量为76000的条带消失了。在这种情况下，正常BALB/c红细胞的次要放射性碘化蛋白质（表观分子量为65000、57000、48000、38000和32000）在未感染和感染的红细胞上均被一系列表观分子量为60000、50000、43000和28000的条带所取代。这些接种剂量的差异可能与这些感染的不同持续时间、贫血的发展以及红细胞表面病理变化的程度有关。约氏疟原虫感染导致感染血液中大多数（如果不是全部）红细胞上的唾液酸糖蛋白的过碘酸盐依赖性标记显著减少。无论有无神经氨酸酶处理，半乳糖氧化酶依赖性糖蛋白标记也大幅下降。红细胞膜糖蛋白上这些碳水化合物基团的变化可能与某些疟疾感染期间未感染和感染红细胞的过度损失有关。
