Schatz C, Jeanblanc B, Offner M
Nouv Presse Med. 1980 Nov 22;9(44):3339-42.
The risk of atheroma can be assessed and valid epidemiological surveys can be carried out by measuring cholesterol in serum high density lipoprotein fraction (HDL) and calculating the HDL cholesterol: VLDL + LDL ratio. This was done in 39 patients free from surgically confirmed atheromatous lesions and in 51 patients presenting with such lesions. Of the four different techniques used for separation in these patients (ultracentrifugation, precipitation with heparin-Mn2+, precipitation with phosphotungstate-Mg2+ and electrophoresis), precipitation with phosphotungstate-Mg/2+ seems to be the most suitable, since there is no degradation of the HDL fraction as during electrophoresis on polyacrylamide gel, and less floculation of the supernatant after separation. Contrary to ultracentrifugation, which requires sophisticated equipment and good technical skill, the technique is easily carried out.
通过测量血清高密度脂蛋白（HDL）部分的胆固醇并计算HDL胆固醇与极低密度脂蛋白（VLDL）加低密度脂蛋白（LDL）的比值，可以评估动脉粥样硬化的风险，并开展有效的流行病学调查。对39例经手术确诊无动脉粥样硬化病变的患者以及51例有此类病变的患者进行了上述操作。在这些患者中用于分离的四种不同技术（超速离心、用肝素 - Mn²⁺沉淀、用磷钨酸 - Mg²⁺沉淀和电泳）中，用磷钨酸 - Mg²⁺沉淀似乎是最合适的，因为不像在聚丙烯酰胺凝胶电泳期间HDL部分不会降解，并且分离后上清液的絮凝较少。与需要精密设备和良好技术技能的超速离心相反，该技术易于实施。
