Baer R, Dubin D T
Nucleic Acids Res. 1980 Nov 11;8(21):4927-41. doi: 10.1093/nar/8.21.4927.
The 220 3'-terminal nucleotides of the small ribosomal subunit RNA (13S) of hamster (BHK-21) cell mitochondria have been sequenced and the positions of post-transcriptionally methylated residues within this sequence have been established. Also, we have derived the secondary structure of the 3'-terminus of mitochondrial 13S rRNA by 1) searching nucleotide sequences of 13S rRNA, procaryotic 16S rRNA and eucaryotic 18S rRNA for common secondary structures and 2) using single-strand specific endonucleases to map secondary interactions in 13S rRNA. The pyrimidine tract CCUCC in E. coli 16S rRNA, which participates in base-pairing with bacterial mRNA, is absent in mitochondrial 13S rRNA. We believe that the binding of mRNA to mammalian mitochondrial ribosomes is not mediated by a conventional Shine-Dalgarno interaction.
已对仓鼠（BHK - 21）细胞线粒体小核糖体亚基RNA（13S）的220个3'末端核苷酸进行了测序，并确定了该序列中转录后甲基化残基的位置。此外，我们通过以下方式推导了线粒体13S rRNA 3'末端的二级结构：1）在13S rRNA、原核16S rRNA和真核18S rRNA的核苷酸序列中搜索共同的二级结构；2）使用单链特异性核酸内切酶来绘制13S rRNA中的二级相互作用。大肠杆菌16S rRNA中参与与细菌mRNA碱基配对的嘧啶序列CCUCC在线粒体13S rRNA中不存在。我们认为，mRNA与哺乳动物线粒体核糖体的结合不是由传统的Shine - Dalgarno相互作用介导的。
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