Palmero I, Renart J, Sastre L
Instituto de Investigaciones Biomédicas del CSIC, Universidad Autónoma de Madrid, Spain.
Gene. 1988 Sep 7;68(2):239-48. doi: 10.1016/0378-1119(88)90026-1.
cDNA clones coding for Artemia mitochondrial 16S ribosomal RNA (rRNA) have been isolated. The clones cover from nucleotide 650 of the RNA molecule to its 3' end. The comparison of Artemia sequence with both vertebrate and invertebrate mitochondrial 16S rRNA sequences has shown the existence of regions of high similarity between them. A model for the secondary structure of the 3' half of Artemia mitochondrial 16S rRNA is proposed. The size of the rRNA molecule has been estimated at 1.35 kb. Despite the similarity of the Artemia gene to insect rRNA in size, sequence and secondary structure, the G + C content of the Artemia gene (42%) is closer to that of mammals than to the insect genes. The number of mitochondria in Artemia has been estimated at 1500 per diploid genome in the cyst and 4000 in the nauplius. In contrast, the amount of mt 16S rRNA is constant at all stages of Artemia development.
编码卤虫线粒体16S核糖体RNA（rRNA）的cDNA克隆已被分离出来。这些克隆覆盖了RNA分子从第650个核苷酸到其3'端的区域。卤虫序列与脊椎动物和无脊椎动物线粒体16S rRNA序列的比较表明，它们之间存在高度相似的区域。提出了卤虫线粒体16S rRNA 3'端一半的二级结构模型。rRNA分子的大小估计为1.35 kb。尽管卤虫基因在大小、序列和二级结构上与昆虫rRNA相似，但其G + C含量（42%）与哺乳动物的更接近，而与昆虫基因的差异较大。据估计，卤虫每个二倍体基因组中的线粒体数量在囊胚期为1500个，在无节幼体期为4000个。相比之下，mt 16S rRNA的量在卤虫发育的所有阶段都是恒定的。
