Mettler L, Ehrig G, Volkmann J
Res Exp Med (Berl). 1980;177(3):255-61. doi: 10.1007/BF01851216.
The present paper describes the influence of antibodies against spermatozoa on the in vitro fertilization of inbred mice. "Antibodies against spermatozoa' were obtained by immunization in the syngeneic and allogeneic system of C57BL/J Hand and C3H/2He female mice with washed spermatozoa of the corresponding male partners. These antibodies were added to the in vitro culture. The resulting fertilization rates were compared with control cultures where sera of non-immunized and erythrocytes sensitized mice were added. For comparison the same experiments were performed with isolated gamma-globulin fractions of the antisera. The influence of the antibodies against spermatozoa was deomonstrated by the reduction of the cleavage rate (1-2% cleaved oocytes) in comparison to the normally acheived fertilization rate of about 50%. In addition, the oocytes of the experimental group revealed shrinking of the cell mass in all cases.
本文描述了抗精子抗体对近交系小鼠体外受精的影响。“抗精子抗体”是通过用相应雄性伴侣洗涤过的精子在C57BL/JH和C3H/2He雌性小鼠的同基因和异基因系统中进行免疫获得的。将这些抗体添加到体外培养物中。将所得的受精率与添加未免疫小鼠血清和红细胞致敏小鼠血清的对照培养物进行比较。为了进行比较，用抗血清的分离γ-球蛋白组分进行了相同的实验。与正常约50%的受精率相比，抗精子抗体的影响通过卵裂率降低（1-2%的卵母细胞发生卵裂）得以证明。此外，实验组的卵母细胞在所有情况下均显示细胞团缩小。
