Samuelson K, Eklund A
Scand J Clin Lab Invest. 1980 Oct;40(6):555-61. doi: 10.3109/00365518009091964.
Radioimmunoassays were developed for the determination of urinary unsulphated and total cholic and chenodeoxycholic acid conjugates. For the determination of unsulphated bile acid conjugates the urine was assayed directly after adjustment of pH to 7.4. The total conjugates were determined after extraction of the bile acids on an XAD-2 column, solvolysis of the sulphated bile acids and purification on a second XAD-2 column. Comparison of the results obtained using radioimmunoassay with those obtained using gas chromatography on twenty-two urine samples from patients with different types of liver disease and on ten urine samples from healthy subjects indicated a good correlation.
已开发出放射免疫分析法用于测定尿中未硫酸化的、总胆酸和鹅去氧胆酸结合物。对于未硫酸化胆汁酸结合物的测定，将尿液pH调至7.4后直接进行分析。总结合物的测定是在胆汁酸于XAD - 2柱上萃取、硫酸化胆汁酸溶剂解以及在第二根XAD - 2柱上纯化之后进行的。对22份来自不同类型肝病患者的尿液样本以及10份来自健康受试者的尿液样本，使用放射免疫分析法所得结果与使用气相色谱法所得结果进行比较，结果显示具有良好的相关性。
