Sato T, Oguma S, Saito T, Yoshinaga K
Tohoku J Exp Med. 1980 Oct;132(2):207-12. doi: 10.1620/tjem.132.207.
A method was established to isolate the glomeruli from the kidney fixed in formaldehyde. O-Hydroxylysylglycosides and hydroxyproline in the isolated glomeruli from the kidney of normal subjects, diabetics and subjects with chronic glomerulonephritis were measured on the alkaline hydrolyzate of the glomeruli. O-Hydroxylysylglycosides in 1000 glomeruli were 9.42 +/- 2.68 nmoles (mean +/- S.D.) for controls, 12.92 +/- 7.36 nmoles for diabetics and 12.54 +/- 4.62 nmoles for subjects with chronic glomerulonephritis. Hydroxyproline in 1000 glomeruli from control, diabetics and subjects with chronic glomerulonephritis was 43.0 +/- 10.7, 82.0 +/- 39.3 and 52.1 +/- 21.3 nmoles, respectively. Hydroxyproline in the hyalinized glomeruli from diabetics was 123.3 +/- 22.4 nmoles/1000 glomeruli, and significantly increased in comparison with that in the glomeruli from chronic glomerulonephritis or control subjects.
建立了一种从甲醛固定的肾脏中分离肾小球的方法。对正常受试者、糖尿病患者和慢性肾小球肾炎患者肾脏分离出的肾小球碱性水解产物中的O-羟赖氨酰糖苷和羟脯氨酸进行了测定。对照组1000个肾小球中的O-羟赖氨酰糖苷为9.42±2.68纳摩尔（平均值±标准差），糖尿病患者为12.92±7.36纳摩尔，慢性肾小球肾炎患者为12.54±4.62纳摩尔。对照组、糖尿病患者和慢性肾小球肾炎患者1000个肾小球中的羟脯氨酸分别为43.0±10.7、82.0±39.3和52.1±21.3纳摩尔。糖尿病患者玻璃样变肾小球中的羟脯氨酸为123.3±22.4纳摩尔/1000个肾小球，与慢性肾小球肾炎患者或对照组肾小球中的羟脯氨酸相比显著增加。
