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[从含有培养弧菌的固体培养基中提取霍乱毒素的方法]

[Method of extracting cholera toxin from solid media containing cultured vibrios].

作者信息

Efremenko V I, Vladimtseva I V, Durikhin K V, Popova A E, Dolmatova L A

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1980 Nov(11):68-71.

PMID:7445852
Abstract

The possibility of obtaining biologically active cholerogen by extracting it from solid culture media used for the cultivation of vibrios is shown. V. cholerae, strain 569B, cultivated on solid media produced about 3 times more toxin per 1 ml of the culture medium than during the process of its cultivation with the use of liquid culture media. The inculation temperature proved to have no essential influence on the toxin production by vibrios.

摘要

研究表明,从用于培养弧菌的固体培养基中提取生物活性霍乱原是可能的。在固体培养基上培养的霍乱弧菌569B菌株,每1毫升培养基产生的毒素比使用液体培养基培养时多约3倍。结果证明,接种温度对弧菌产生毒素没有实质性影响。

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