用于霍乱弧菌O1生物型埃尔托生物型生产霍乱毒素的新培养基。
New medium for the production of cholera toxin by Vibrio cholerae O1 biotype El Tor.
作者信息
Iwanaga M, Yamamoto K
出版信息
J Clin Microbiol. 1985 Sep;22(3):405-8. doi: 10.1128/jcm.22.3.405-408.1985.
A new medium that stimulates in vitro production of cholera toxin by Vibrio cholerae O1 El Tor (El Tor vibrios) was developed. The medium contains 0.5% NaCl, 0.3% NaHCO3, 0.4% yeast extract, and 1.5% Bacto-Peptone. El Tor vibrios were cultured in a stationary test tube at 37 degrees C for 20 h. The culture supernatant was assayed for cholera toxin by a reversed passive latex agglutination method. Most vibrios grown in this medium produced 10 to 20 times more toxin than in traditional syncase medium. The number of live vibrios at the end of culture was about 10(8)/ml in the new medium (AKI medium) and about 10(10)/ml in the syncase medium. As a result, each individual organism in the new medium should have produced as much as 1,000 times more toxin than in syncase medium. Sodium bicarbonate was found to be the most important factor in toxin production by El Tor vibrios in the new medium. We recommend this new medium because of its high yield of cholera toxin and its technical simplicity.
一种能刺激霍乱弧菌O1 El Tor型（El Tor弧菌）体外产生霍乱毒素的新培养基被研制出来。该培养基含有0.5%氯化钠、0.3%碳酸氢钠、0.4%酵母提取物和1.5%细菌蛋白胨。El Tor弧菌在固定的试管中于37℃培养20小时。通过反向被动乳胶凝集法检测培养上清液中的霍乱毒素。在此培养基中生长的大多数弧菌产生的毒素比在传统的同步酶培养基中多10至20倍。培养结束时，新培养基（AKI培养基）中活弧菌的数量约为10⁸/ml，同步酶培养基中约为10¹⁰/ml。因此，新培养基中每个个体产生的毒素应该比同步酶培养基中多1000倍。发现碳酸氢钠是新培养基中El Tor弧菌产生毒素的最重要因素。由于其霍乱毒素产量高且技术简单，我们推荐这种新培养基。
Zotero 插件