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[细菌学和血清学研究在儿童肠道耶尔森菌病检测中的诊断价值]

[Diagnostic value of bacteriologic and serologic studies in detecting intestinal yersiniosis in children].

作者信息

Koroliuk A M, Strelkova M R, Avramenko V S

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1980 Jun(6):19-23.

PMID:7445857
Abstract

The survey of 200 children with diarrhea revealed the presence of enteric yersiniosis in 31 children (15.5%). Y. enterocolitica culture was isolated from the feces of 17 children (8.5%), diagnostic serological shifts were detected in 27 children (13.5%). Among 100 children hospitalized with diarrhea of previously established etiology (dysentery, salmonellosis, etc) 6 children were found to have yersiniosis. The survey of 100 practically healthy children did not reveal any cases of enteric yersiniosis or healthy carriership. To isolate Yersinia, the feces were incubated in phosphate buffer in the cold (4--6 degrees C) and then inoculated into common diagnostic media for enterobacteria. Antibodies were detected in the indirect hemagglutination test with the use of dried erythrocytic yersiniosis diagnostic reagent. A tentative diagnostic titer of 1 : 200 was determined. Antibodies to the causative agent appeared on the 1st week, reached the maximum level on the 2nd and then gradually decreased. The clinical symptoms of the enteric form of yersiniosis resemble those of other kinds of infectious diarrhea, non-dysenteric in etiology. The authors believe that it is necessary for all children with diarrhea, especially at the age of 1--7 years, to be examined for enteric yersiniosis.

摘要

对200名腹泻儿童的调查显示,31名儿童(15.5%)存在肠道耶尔森菌病。从17名儿童(8.5%)的粪便中分离出小肠结肠炎耶尔森菌培养物,在27名儿童(13.5%)中检测到诊断性血清学变化。在100名因先前确定病因(痢疾、沙门氏菌病等)的腹泻而住院的儿童中,发现6名儿童患有耶尔森菌病。对100名实际健康儿童的调查未发现任何肠道耶尔森菌病病例或健康带菌者。为分离耶尔森菌,将粪便在磷酸盐缓冲液中于低温(4 - 6摄氏度)下孵育,然后接种到用于肠道杆菌的普通诊断培养基中。使用干燥红细胞耶尔森菌病诊断试剂通过间接血凝试验检测抗体。确定初步诊断滴度为1:200。针对病原体的抗体在第1周出现,在第2周达到最高水平,然后逐渐下降。肠道型耶尔森菌病的临床症状与其他病因非痢疾性的感染性腹泻相似。作者认为,所有腹泻儿童,尤其是1至7岁的儿童,都有必要进行肠道耶尔森菌病检查。

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Zh Mikrobiol Epidemiol Immunobiol. 1980 Jun(6):19-23.
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