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非离子型去污剂对从大鼠肝脏和兔心脏膜中提取磷脂和蛋白质的作用。

The action of non-ionic detergents on the extraction of phospholipids and proteins from rat liver and rabbit heart membranes.

作者信息

Thang N X, Farkas T, Wollemann M

出版信息

Acta Biochim Biophys Acad Sci Hung. 1980;15(3):205-9.

PMID:7445965
Abstract

Triton X-100 and Lubrol PX at similar concentrations extracted more phospholipid than protein from rat liver and rabbit heart membranes, as compared with their original protein and phospholipid contents, respectively. All the types of membrane phospholipid were solubilized from rat liver membrane with the used non ionic detergents, but less sphingomyelin was extracted than phosphatidylcholine and phosphatidylethanolamine.

摘要

与大鼠肝脏和兔心脏膜的原始蛋白质和磷脂含量相比,相似浓度的曲拉通X-100和聚氧乙烯月桂醚从这些膜中提取的磷脂比蛋白质更多。使用的非离子去污剂能使大鼠肝膜中的所有类型膜磷脂溶解,但提取的鞘磷脂比磷脂酰胆碱和磷脂酰乙醇胺少。

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