Kisieliński T, Gajewski D, Gidyńska T, Owczarczyk H
Acta Physiol Pol. 1980 May-Jun;31(3):279-88.
The reported investigations were carried out on female Wistar rats receiving chlorphenvinphos and phospholine in doses equalling 3/4 LD50 onto the skin and intragastrically. the levels of acetylcholinesterase (AChE) were determined in the erythrocytes, liver and tibialis muscle and in three brain areas: pontomedullary area, hypothalamus and cerebral cortex. The activity of the enzyme was determined 15 and 30 minutes, and 3, 24 and 72 hours after poisoning. A high degree of AChE inhibition was observed in the brain after chlorphenvinphos poisoning (activity fall to 15--30%) and a slight decrease of AChE activity after administration of phospholine independently of the route. After oral poisoning with both preparations the AChE activity level in the erythrocytes was from about 27% to 31%, in the liver from 18 to 23%, and in the skeletal muscle from 51 to 54%, while with the transcutaneous route of poisoning these values were: 18--23%, 41--52%, and 36--48% respectively. A high degree of enzyme restoration was observed during 72 hours of observation. AChE activity was then 60--80% of the initial value.
所报道的研究是在雌性Wistar大鼠身上进行的，这些大鼠经皮肤和经胃给予敌百虫和保棉磷，剂量相当于3/4的半数致死剂量（LD50）。测定了红细胞、肝脏、胫骨前肌以及脑的三个区域（脑桥延髓区、下丘脑和大脑皮层）中的乙酰胆碱酯酶（AChE）水平。在中毒后15分钟和30分钟、3小时、24小时和72小时测定该酶的活性。敌百虫中毒后在脑中观察到高度的AChE抑制（活性降至15%-30%），而给予保棉磷后，无论给药途径如何，AChE活性均略有下降。经口给予这两种制剂中毒后，红细胞中的AChE活性水平约为27%至31%，肝脏中为18%至23%，骨骼肌中为51%至54%，而经皮中毒途径时，这些值分别为18%-23%、41%-52%和36%-48%。在72小时的观察期内观察到该酶有高度的恢复。此时AChE活性为初始值的60%-80%。
