Gajewski D
Acta Physiol Pol. 1980 Sep-Oct;31(5):575-80.
The reported investigations were carried out on female Wistar rats aged about 6 weeks, weighing 150 g. During 28 days the fats were given daily organophosphates (DFP, DDVP, Chlorfenvinphos, IPO-62, IPO-63, Phospholine) in a dose of 1/10 LD50 subcutaneously. The observations were conducted on groups of 8 rats. The body weight of the animals was noted on the 10th, 20th and 28th day of the experiment. On the 28th day the rats were killed and the activity of acetylcholinesterase (AChE) was determined by the biochemical method of Hestrin in liver homogenate and in the microsomal, mitochondrial and soluble fractions of liver cells. After 28 days of the experiment the body weight of the rats poisoned with these substances amounted to from 80.8% (DFP) to 90.7% (IPO-63) of that in control animals. The AChE activity was also reduced in relation to the control group ranging in the liver homogenate from 49.7% (DFP) to 75.6% (IPO-63), in the microsomal fraction from 33.0% (DFP) to 63.8% (IPO-63), in the mitochondrial fraction from 45.5% (DFP) to 72.9% (IPO-63), and in the soluble fraction from 52.8% (DFP) to 80.5% (DDVP).
所报道的研究是在约6周龄、体重150克的雌性Wistar大鼠身上进行的。在28天内，每天给这些大鼠皮下注射剂量为1/10 LD50的有机磷酸酯（二异丙基氟磷酸酯、敌敌畏、毒虫畏、IPO - 62、IPO - 63、保棉磷）。观察是在每组8只大鼠身上进行的。在实验的第10天、第20天和第28天记录动物的体重。在第28天处死大鼠，通过赫斯特林生化方法测定肝匀浆以及肝细胞微粒体、线粒体和可溶性部分中的乙酰胆碱酯酶（AChE）活性。实验28天后，用这些物质中毒的大鼠体重相当于对照动物体重的80.8%（二异丙基氟磷酸酯）至90.7%（IPO - 63）。与对照组相比，乙酰胆碱酯酶活性也有所降低，在肝匀浆中从49.7%（二异丙基氟磷酸酯）至75.6%（IPO - 63），在微粒体部分从33.0%（二异丙基氟磷酸酯）至63.8%（IPO - 63），在线粒体部分从45.5%（二异丙基氟磷酸酯）至72.9%（IPO - 63），在可溶性部分从52.8%（二异丙基氟磷酸酯）至80.5%（敌敌畏）。
