Enders A C, Schlafke S, Welsh A O
Am J Anat. 1980 Sep;159(1):59-72. doi: 10.1002/aja.1001590106.
Fixed uteri from rats on the afternoon of day 6 of pregnancy were split to expose the implantation chambers, their enclosed blastocysts, and the imprints of the blastocyst on the adjacent epithelium of the chamber. Some of the implantation chambers were prepared for scanning electron microscopy; other chambers were treated with colloidal iron hydroxide, with cationized ferritin, or with the tannic acid method, and subsequently were prepared for transmission electron microscopy. In this manner, the disposition of the surface-coat markers on the surface of the blastocysts, surface of the uterus within the chamber, and the surface of the uterus that had been apposed to a blastocyst were compared. Despite the pronounced morphological differences between the microvilli of the uterine luminal epithelium in the imprint and those in the rest of the chamber, the binding of the markers was remarkably similar. No evidence of removal of surface coat could be found in that area of the uterus in contact with the blastocyst. In addition, in two instances in the cationized ferritin-treated material, and in another instance in tannic acid-stained material, regions of the apparently adhering trophoblastic cell membranes and uterine cell membranes had abundant coat materials and, possibly, even secretory materials interposed. When blastocyst-sized glass beads were introduced into uteri from animals made pseudopregnant or unilaterally pregnant, the beads failed to elicit a decidual response and made an imprint that did not resemble the imprint of a blastocyst in an implantation chamber. It was concluded that, at least in the initial stages of adhesion, the blastocyst does not bring about a physical removal of the demonstrable aspects of the surface coat of the uterus. It was concluded further, that glass beads are not a suitable object for mimicking a blastocyst in the rat uterus.
妊娠第6天下午处死的大鼠固定子宫被剖开，以暴露着床腔、腔内封闭的囊胚以及囊胚在腔相邻上皮上的印记。部分着床腔用于扫描电子显微镜观察；其他腔用氢氧化铁胶体、阳离子铁蛋白或单宁酸法处理，随后用于透射电子显微镜观察。通过这种方式，比较了囊胚表面、腔内子宫表面以及曾与囊胚贴附的子宫表面上表面涂层标记物的分布情况。尽管印记处子宫腔上皮微绒毛与腔其余部分的微绒毛在形态上有明显差异，但标记物的结合却非常相似。在与囊胚接触的子宫区域未发现表面涂层被去除的迹象。此外，在阳离子铁蛋白处理的材料中有两例，在单宁酸染色的材料中有一例，明显粘连的滋养层细胞膜和子宫细胞膜区域有丰富的涂层物质，甚至可能有分泌物质夹杂其中。当将囊胚大小的玻璃珠植入假孕或单侧妊娠动物的子宫时，玻璃珠未能引发蜕膜反应，其形成的印记与着床腔中囊胚的印记不同。得出的结论是，至少在黏附的初始阶段，囊胚不会导致子宫表面涂层可显示部分的物理性去除。进一步得出的结论是，玻璃珠不是模拟大鼠子宫中囊胚的合适物体。
